Saudi Arabia is the custodian for 2 of the 3 holy mosques for almost 2 billion Muslims worldwide. Each year, pre-COVID, more than 10 million pilgrims come into Saudi Arabia for Hajj and Umrah mainly via Jeddah Airport, which serves as the major international port of entry for pilgrims entering Saudi Arabia. The sheer number of international travelers entering and leaving the country meant that Saudi Arabia is especially susceptible to cross-border dissemination of undesirable microbial pathogens. Active biosurveillance to pre-empt the emergence of microbial disease outbreaks arising from global travels would strengthen the Kingdom’s defense against disease outbreak and epidemics. Here, in collaboration with NoorDx, we demonstrate a biosurveillance workflow that involves wastewater sampling from lavatory tanks of aircrafts at Jeddah International Airport, and omics-based sequencing. Our effort aims to demonstrate the ability to monitor for a wide suite of microbial contaminants associated with global travellers, which would facilitate relevant stakeholders to devise appropriate intervention measures that can minimize unintended dissemination of microbial threats.