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Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing

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Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing

by Chongwei Bi, Gerardo Ramos-Mandujano, Yeteng Tian, Sara Mfarrej, Andri Rachmadi, Sharif Hala, Jinna Xu, Fadwa S Alofi, Asim Khogeer, Anwar M Hashem, Naif AM Almontashiri, Concepcion Rodriguez Esteban, Estrella Nuñez Delicado, Juan Carlos Izpisua Belmonte, Arnab Pain, Mo Li
Year: 2021

Abstract

Strategies for monitoring the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are crucial for combating the pandemic. Detection and mutation surveillance of SARS-CoV-2 and other respiratory viruses require separate and complex workflows that rely on highly specialized facilities, personnel, and reagents. To date, no method can rapidly diagnose multiple viral infections and determine variants in a high-throughput manner.

Keywords

SARS-CoV-2 nanopore sequencing influenza wastewater surveillance co-infection real-time pathogen detection variant virus sequencing mutation surveillance adenovirus isothermal amplification